青年科学工作者论坛2006年第3期

孙长颢  周晓蓉  赵丹

哈尔滨医科大学公共卫生学院，哈尔滨  150086

 

摘要：目的  通过研究共轭亚油酸(CLA)对饮食诱导胰岛素抵抗大鼠脂肪酸连接蛋白（ap2）基因表达的影响，探讨CLA抗糖尿病作用的机制。方法  选用雄性Wistar大鼠，随机分为对照组、高脂组、高脂+CLA组（每100g饲料含CLA分别为0.75g、1.50g、3.00g），每组动物10只，观察CLA对胰岛素抵抗大鼠胰岛素、血糖水平的影响，并应用RT-PCR的方法检测ap2、过氧化物酶体增殖物活性受体γ（PPARγ）的表达水平。结果  高脂组大鼠血清游离脂肪酸（FFA）、胰岛素和糖血水平显著高于基础组，CLA 可降低胰岛素抵抗大鼠血清FFA、血糖、胰岛素水平，并可增加其脂肪组织ap2、PPARγ mRNA的表达水平。结论  CLA可通过激活PPARγ上调脂肪酸连接蛋白基因的表达，改善肥胖大鼠的胰岛素抵抗。

关键词：脂肪酸连接蛋白  过氧化物酶体增殖物激活受体γ  胰岛素抵抗

 

School of Public Health, Harbin Medical University,Harbin 150086,China

 

Abstract:  Objective  To study the effect of conjugated linoleic acid on gene expression of fatty acid binding proteins（ap2）in white obesity tissue of  rats with insulin resistance, and explore the mechanism of resisting diabetes by CLA. Methods  Male Wistar rats were randomly separated to control group, high-fat group and high fat + CLA group(0.75g,1.50g,3.00g% by diet weight), Using reverse transcription polymerse chain reaction (RT-PCR) technique to measure the expression level of ap2 and Peroxisome proliferators-activated receptor－γ(PPARγ) mRNA expression. Results  The FFA, serum insulin and glucose levels of rats with insulin resistance were higher than that of rats fed with basic diet, supplement of CLA can decrease FFA, serum insulin and glucose levels of rats with insulin resistance, and CLA can  increase the expression of ap2 and PPARγ mRNA in adipose tissue of rat with insulin resistance. Conclusion  CLA can improve insulin resistance of obese rat and increase the expression of ap2 mRNA ,possibly acting through activing PPARγ.

Key words: ap2，PPARγ，insulin resistance

 

 

基金项目：国家自然科学基金资助项目（No.30271118）

作者简介：孙长颢，男，教授，博士研究生导师，哈尔滨医科大学公共卫生学院院长

中国疾病预防控制中心营养与食品安全所，北京  100050

 

摘要：目的 了解2000年中国不同地区膳食中总汞含量和成年男子人均汞膳食摄入量。方法采用总膳食研究方法，通过膳食调查、统计不同地区人群各种食物的消费量、按照所得的食物消费量数据烹调加工成可食的各种食物类别。膳食样品中总汞含量采用冷原子吸收光谱法测定，并按成年男子各种食品的消费量与实测膳食中汞的含量相乘得到不同地区总汞的实际摄入量。采用世界卫生组织推荐的总汞每周可耐受摄入量（PTWI）值评价中国四个大区成年男子膳食中总汞摄入的安全性。结果 除某些地区的个别食物样品超过中国食品卫生中总汞限量标准外，四个大区和全国成年男子平均膳食总汞摄入量（占%PTWI）分别为：8.86µg (19.7%)、4.69µg (10.4%)、7.01µg (15.6%)、6.88µg (15.3%)和6.86µg (15.2%)。结论  结果显示中国人群膳食中总汞摄入量是安全的。

关键词：中国总膳食研究  膳食汞摄入量  膳食营养

 

National Institute for Nutrition and Food Safety, Chinese Center for Disease Control and Prevention，Beijing 100050, China

 

Abstract：Objective  To assess the safety of dietary mercuric intakes in different areas in China. Methods  Using the Chinese total dietary study method, the analytical samples were obtained in different areas by food consumption survey, food aggregation, food sampling and preparation. The levels of dietary total mercury were determined by cold vapor atomic absorption spectrometry. The dietary total mercuric intakes in different areas were obtained by timing the food consumption data and the mercuric content in different dietary samples. The safety of dietary mercury was evaluated in Chinese four regions and average adults by using the dietary mercuric PTWI recommended by WHO. Results  The results indicate that the dietary mercuric intakes are safe in different regions. Only a few samples in some areas exceed tolerance limits of China national standard. Dietary total mercuric intakes (of PTWI) in four different regions adults and mean were 8.86µg (19.7%), 4.69µg (10.4%), 7.01µg (15.6%), 6.88µg (15.3%), 6.86µg (15.2%), respectively. Mainly sources of dietary mercuric intake were cereals, vegetables and sea foods. Conclusion  The result shows that the total mercuric intakes in Chinese people are safe.

Key words: Chinese total diet study, dietary mercuric intakes

 

 

 

基金项目：科学技术部科技基础性工作项目基金

作者简介：李筱薇，女，助理研究员，中国协和医科大学公共卫生学院硕士研究生
文章编号：1000-8020（2006）03-0357-03

刘跟生　徐贵发

山东大学公共卫生学院营养与食品卫生研究所，济南  250012

摘要：单不饱和脂肪酸（MUFA）是脂肪酸的一种，其碳链上只有一个双键。研究发现MUFA能够正向调节血脂代谢，降低低密度脂蛋白胆固醇（LDL）的氧化敏感性，保护血管内皮和降低血液高凝状态。本文将从以上几个方面介绍MUFA对心血管的保护作用。

关键词：单不饱和脂肪酸  血脂  内皮  心血管

 

Astract: Monounsaturated fatty acid （MUFA） is a sort of fatty acids, including a double bond in its carbon chain. Substantial evidence exists to suggest that MUFA can positively regulate the metabolism of serum lipids, decrease the susceptibility of LDL to oxidative modification, protect the artery endothelium, and decrease prothrombotic environment. The protective effect  of MUFA to the cardiovascular system was introduced in the paper.

Key words：monounsaturated fatty acid, serium lipids, endothelium, cardiovascular system

 

作者简介：刘跟生，男，硕士研究生，E-mail：nutritiongen@163.com