青年科学工作者论坛2005年第5期

杨年红 王重建 许明佳 胡学锋 郝丽萍 刘烈刚 孙秀发
华中科技大学同济医学院公共卫生学院营养与食品卫生系，武汉 430030

摘要：
　　目的 探讨白色脂肪组织UCPs和PPARγ2基因在高脂饮食诱导大鼠肥胖抵抗中的作用。
　　方法 36只雌性SD大鼠，按体重随机分为高脂实验组和基础对照组，分别给予高脂饲料和基础饲料13周。实验结束时，根据体重将高脂组大鼠分为饮食诱导肥胖(DIO)和饮食诱导肥胖抵抗 (DIO-R)大鼠，比较各组大鼠体重、脂肪湿重、脂体比、空腹血糖(FBG)及血脂谱；RT-PCR法测定白色脂肪组织UCP2、UCP3及PPARγ2 mRNA的表达水平。
　　结果 DIO-R及DIO大鼠脂体比、TC、LDL-C、TG等指标均显著高于对照组相，但DIO-R组体重、脂肪湿重、脂体比、TG显著低于DIO组(P<0.05)； DIO-R大鼠UCP2和UCP3 mRNA的表达水平高于DIO大鼠和对照组(P<0.01)，PPARγ2 mRNA的表达水平低于DIO大鼠(P<0.01)，高于对照组(P<0.01)。结论 高脂饮食诱导大鼠发生肥胖抵抗与白色脂肪组UCP2、UCP3高表达及PPARγ2 mRNA表达下降密切相关。

关键词：高脂饮食 肥胖抵抗 解偶联蛋白 过氧化物酶体增值物激活受体γ2
中图分类号：R1511 文献标识码：A

UCPs and PPARγ2 mRNA in diet induced obesity resistant rats

Yang Nian-hong, Wang Chong-jian, Xu Ming-jia, Hu Xue-feng, et al.

Department of Nutrition and Food Hygiene, School of Public Health, Tongji Medical College,Huazhong University of Science and Technology, Wuhan 430030, China

Abstract：Objective To explore the expression of uncoupling proteins (UCP) and peroxisome proliferator activated receptorγ2 (PPARγ2) mRNA in white adipose of highfat diet induced obesity resistant rats. Methods Thirtysix female SD rats were randomly divided into highfat diet group (n=27) and chow fed control group (n=9), and given either highfat diet or chow for thirteen weeks. Then the highfat diet group was subdivided into Dietary Induced Obesity (DIO) and Dietary Induced Obesity Resistant (DIOR) group according to the final body weight. Basic parameters, fasting blood glucose (FBG), blood lipid levels were measured and compared. Reverse transcriptionpolymerase chain reaction (RT-PCR) was used to measure the expression of UCP2, UCP3, and PPARγ2 mRNA in white adipose. Results Adipose deposits, body fat percent, TC, and TG conceration of both DIO-R and DIO groups were significantly higher than those in control group, while body weight，body fat percent and TG level were significantly lower in DIO-R than DIO rats. The expression of UCP2 and UCP3 mRNA in white adipose of DIO-R rats were significantly higher than those of DIO and control group. The expression of PPARγ2 mRNA in white adipose of DIO-R group was significantly lower than that in DIO group. Conclusion Highfat diet induced obesity resistance were closely associated with the increased UCP2, UCP3, and decreased PPARγ2 mRNA in white adipose.

Key words:highfat diet， obesity resistance， UCP2, UCP3, PPARγ2

基金项目：国家自然科学基金资助项目（ No.30371213 ）；湖北省卫生厅基金资助项目（ No.LJ200212 ）
作者简介：杨年红，女，副教授，博士研究生

李珂 姚平 周绍良 宋方方 章锡平 孙秀发 刘烈刚1
华中科技大学同济医学院公共卫生学院营养与食品卫生学系,武汉 430030

摘要：
　　目的 研究银杏叶提取物(EGB)对酒精所致大鼠睾丸氧化损伤的预防保护作用。
　　方法 雄性SD大鼠30％酒精灌胃(2.37g/kg bw)前，EGB采用分组(低剂量组48μg/g bw和高剂量组96μg/g bw)预防性给药的方法。于实验90d检测大鼠睾丸组织中超氧化物歧化酶(SOD)、谷胱甘肽转移酶(GST)、谷胱甘肽过氧化物酶(GSH-Px)、过氧化氢酶(CAT)、和谷胱甘肽(GSH)、活性氧(ROS)以及丙二醛(MDA)的含量。通过亚细胞分离方法提取大鼠睾丸微粒体，测定微粒体血红素氧化酶-1(HO-1)活性。采用RT-PCR检测睾丸组织中血红素氧化酶-1(HO-1)mRNA表达水平。
　　结果 慢性酒精摄入90d后，EGB组睾丸匀浆ROS、MDA较酒精组有明显降低(P<0.05)；相反， GST、G-Px、CAT、SOD、HO-1活性以及GSH均有显著性升高(P<0.05)；EGB诱导HO-1高表达。结论 长期慢性酒精摄入引起大鼠睾丸氧化损伤。EGB可作为一种预防性的抗氧化剂减轻这种氧化损伤；其机制可能与诱导HO1高表达，清除自由基，抑制脂质过氧化反应有关。

关键词：银杏叶提取物 氧化损伤 抗氧化酶 血红素氧化酶-1
中图分类号：R961〓Q579.1 文献标识码：A

Protective effects of extract of ginkgo biloba againstethanol  induced oxidative injury in rat testes

Li Ke, Yao Ping, Zhou Shao-liang, Song Fang-fang, et al.

Department of Nutrition and Food Hygiene, School of Public Health,Tongji MedicalCollege, HuazhongUniversity of Science and Technology, Wuhan 〓 430030, China

Abstract ： Objective This study was designed to investigate whether EGB protect against ethanol induced oxidative injury in rat testes. Methods The rats were pretreated with EGB (4 . 8 ， 9 . 6mg/ 100g bw per day) before 30 ％ ethanol administration ( 2 . 37g /kg bw by gastric incubation). Ninety days later, the rats were killed in order to measure the activities of Superoxide Dismutase(SOD), Glutathione Reductase(GST), Glutathione Peroxidase(GSH - Px), Catalase(CAT) and the contents of Glutathione(GSH),Reactive Oxygen Species(ROS), Malondialdehyde(MDA)in rat testes. The microsomal fractions were obtained by the method of differential centrifugation, and HO - 1(Heme Oxygenase - 1) activity in testicular microsomal was measured. To examine the expression of HO - 1 mRNA by reverse transcription polymerase chain reaction (RT - PCR). Results Compared with ethanol group, ROS, and MDA contents significantly decreased in EGB group testes, respectively(P<0 . 05). GST, GSH  Px, CAT, SOD activities, and GSH content in EGB group testes elevated markedly(P<0 . 05).EGB could enhance HO - 1mRNA expression remarkably. Conclusion It is suggested that EGB as a preventive antioxidant can protect against ethanol  induced testicular injury ， which may be associated with HO - 1 activity enhancement, free radicals elimination, lipid per  oxidation reaction inhibition.

Key words: extracts of Ginkgo biloba(EGB), oxidative injury, antioxidant enzyme, heme oxy Genase - 1(HO - 1)

基金项目：国家自然科学基金项目 (No.30271130)
作者简介：李珂，女，硕士研究生
1 通讯作者

苏丽琴 综述 金银龙 审校
中国疾病预防控制中心环境与健康相关产品安全所，北京 100021

摘要： 慢性砷中毒对人体健康的损害严重而且危害广泛，一直是世界各国广泛关注的问题。本文首先简要介绍了慢性砷中毒的类型和主要临床表现，然后从砷的接触史、临床表现和实验室检查三个方面介绍国内外大规模现场人群调查的研究现状，并讨论这三方面研究对慢性砷中毒判定的意义，最后提出了进一步开展慢性砷中毒人群流行病学调查的几点思考。

关键词： 慢性暴露 砷中毒 流行病学 判定
中图分类号： R181 . 23 R599 . 9 R994 . 6 文献标识码： A

Significance of field epidemiologic study to identificationof chronic arsenic poisoning

Su Li - qin, Jin Yin - long

Institute for Environmental Health and Related Product Safety, Chinese Center for Disease Control and Prevention, Beijing 100021, China

Abstract ： Chronic arsenic poisoning has serious and extensive impact on human health, which attracts wide attention worldwide. Bases on vast public survey, this article introduces recent field studies on chronic arsenic poisoning from three aspects: exposure history, clinical symptoms and laboratory evidences, and also explains the meaning of each index to the determination of chronic arsenic poisoning, then bring forward some considerations on further epidemiological studies on chronic arsenic poisoning.

Key words: chronic exposure, arsenic poisoning, epidemiology, determination

基金项目 ：环境污染对人体健康的影响与损害诊断技术研究
作者简介：苏丽琴，女，硕士研究生