青年科学工作者论坛2009年第1期
锌对生长期大鼠海马Uch2L1 表达的调控作用
刘静 蒋与刚1 ,2 黄承钰 房红芸2 房恒通2 庞伟2
四川大学华西公共卫生学院营养与食品卫生学教研室,成都 610041
摘要:目的 探讨锌对生长期大鼠海马Uch2L1 表达水平的调控作用。方法 雄性断乳Wistar 大鼠36只,按体重随机分为对照组(ZA) 、缺锌组(ZD) 和缺锌对喂组(PF) ,ZD 组喂饲含锌量115mgPkg 的缺锌饲料,PF组和ZA 组喂饲含锌量30mgPkg 的足锌饲料,PF 组摄食量与ZD 组一致。喂饲4 周后,测定血浆锌含量和碱性磷酸酶活性以评价机体锌状况,用避暗试验测定各组大鼠的学习记忆能力,采用Western blot 和RT2PCR 方法检测各组大鼠海马Uch2L1 蛋白及其mRNA 的表达水平。结果 ZD 组血浆锌含量及碱性磷酸酶活性均显著低于PF 组和ZA 组;ZD 组大鼠在避暗试验中的进洞潜伏期显著低于PF 组和ZA 组;与PF 组和ZA 组相比,ZD组大鼠海马Uch2L1 及其mRNA 表达水平明显降低。结论 缺锌可下调大鼠海马Uch2L1 表达水平。
关键词:缺锌 海马 Uch2L1
中图分类号:R15113 Q581 文献标识码:A
Study of zinc on the modulation of Uch2L1 expression in hippocampus of growing rats
LIU Jing , JIANGYugang , HUANG Chengyu , FANG Hongyun , et al .
Department of Nutrition and Food Hygiene , West China School of Public Health , Sichuan University , Chengdu 610041 , China
Abstract : Objective To investigate the effect of zinc on Uch2L1 expression in hippocampus of growing rats.Methods Thirty2six male weanling Wistar rats were randomly assigned to zinc2adequate (ZA) , zinc2deficient (ZD) and pair2fed (PF) groups. Rats of ZD group were given free access to a basal diet (Zn 115mgPkg) . Rats of other two groups were given the basal diet supplemented with zinc sulfate ( Zn 30mgPkg) . PF rats consumed the same intakes as the corresponding ZD partner on the previous day. After 42wk treatment , plasma zinc concentration and alkaline phosphatase activity were tested. Passive avoidance performance was used to examine learning and memory function. Western blot and RT2PCR were used to assess the expressions of Uch2L1 protein and mRNA in rat hippocampus , respectively. Results Zinc deprivation dramatically decreased plasma zinc concentration and alkaline phosphatase activity. Significantly shorter latency
period in passive avoidance performance in ZD group were observed. Uch2L1 protein and mRNA expression levels in hippocampus significantly reduced in comparison with ZA and PF groups. Conclusion Zinc deficiency could induce
downregulation of Uch2L1 expression.
Key words :zinc deficiency , hippocampus , Uch2L1
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基金项目: 国家自然科学基金资助项目( No. 30471466 ,30872098) ;天津自然科学基金资助项目(No. 05YFJMJC05500)
作者简介:刘静,女,博士研究生
1 通讯作者:蒋与刚,男,E2mail :jyg1967 @1261com
2 军事医学科学院卫生学环境医学研究所