黄河三角洲水源性高碘地区分布与居民食用盐现状调查研究 郭晓尉 翟丽屏 刘源 王欣 刘传蛟 秦启亮 陈祖培 1 |
关键词:水碘 高碘地区 碘盐 黄河三角洲 Study on the present status of the areas with high iodine concentration indrinking water and edible salt at household levels in Ohio of Yellow River Guo Xiao - wei, Zhai Li - ping, Liu Yuan, Wang Xin,et al. Shandong Institute for Endemic Disease Control and Research, Jinan 250014, China Abstract : Objective To understand the present condition of iodine excess areas and edible salt at household levels in Ohio of Yellow River , which will provide the evidence to control it. Methods A cross section in one time was adopted for the epidemiological survey based on the east, west, south, north and central in all of townships from 8 counties. 2 samples of drinking water from each village were tested their water iodine content as well as the data regarding to their recourses and the depth of wells. 5 samples of edible salt were collected from each village for quantitative analysis. Results We investigated 451 villages in 92 townships of 8 counties. 800 samples of drinking water were tested which values of iodine content were (110 . 93 ± 152 . 26) μ g/L in main, 55 . 83 μ g/L(0 . 84 , 997 . 82 μ g/L)in medium. 102 . 39 thousand population are at risk for iodine excess and living in 24 townships of 7 counties where iodine concentration is over 150 μ g/L in drinking water, with (327 . 72 ± 192 . 19) μ g/L in mean value or 253 . 87 μ g/L(150 . 78 , 997 . 82 μ g/L)in medium. The rate of iodized salt is 97 . 2%. All the iodine excess areas are located in alluvial plain of Yellow River. The etiology of high iodine in shallow well water may be supposed to be iodine aggregation formed by Yellow River in terms of thousands of flood in thousands of years. But iodine excess in deep well water may be related to rotten, deposit marine living beings rich in iodine millions upon millions years ago. Conclusion There were distinctive features of iodine excess in drinking water from both shallow well and deep well, 24 iodine excess areas in Ohio of Yellow River. It has suggested that iodized salt intervention should be stopped in the areas and starting the health education project, survey of iodized salt in the region. Key words: water iodine, iodine excess areas, iodized salt, Ohio of Yellow River
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何超 樊学军1 汪东篱 刘丽英2 王翠苹2 裴晓方3 |
摘要: 关键词:多重PCR 沙门菌 志贺菌 大肠杆菌O157∶H7 Primarily study on a multiplex PCR based system for the rapid detection of Salmonella spp., Shigella spp. and Escherichia coli O157 ∶ H7 He Chao, Fan Xue - jun, Wang Dong - li, Liu Li - ying, et al. West China School of Public Health, Sichuan University , Chengdu 610041, China Abstract:Objective To establish a multiplex PCRbased system for the simultaneous detection of Salmonella spp., Shigella spp. and Escherichia coli O157∶H7 in 12 hours.Methods After 6 h nonselective enrichment in BPW, DNA template were prepared at 100℃ for 10min. Three sets of primers were designed to amplify the gene segments of invA of Salmonella spp, ipaH of Shigella spp, and uidA of E. coli O157∶H7, and the products were analyzed by electrophoresis. At the same time, this system was optimized,and the specificity and sensitivity of this system were evaluated.Results Three target bacteria were detected in 12 h by using this multiplex PCRbased system. The sensitivity of it was up to 10-30cfu/ml, and the high specificity was demonstrated by detecting 23 target stains and 15 nontarget stains.Conclusion A rapid,specific, and sensitive multiplex PCRbased system for the simultaneous detection of Salmonella spp., Shigella spp. and E. coli O157∶H7 in 12 h has been studied primarily. 基金项目:国家质检总局科研计划项目( No.J2005J0115 ) |