青年科学工作者论坛2005年第6期

黄河三角洲水源性高碘地区分布与居民食用盐现状调查研究

郭晓尉 翟丽屏 刘源 王欣 刘传蛟 秦启亮 陈祖培 1
山东省地方病防治研究所,济南 250014


摘要:
  目的 查清黄河三角洲的水源性高碘地区分布和居民食用盐现状以制定干预策略。
  方法 采用横断面调查方法,每个被调查县的所有乡镇均按东、西、南、北、中五个不同方位抽取5个村,在每个村采集饮用水样2份进行水碘含量检测,并了解饮水源类型及水井深度。每个村随机采集5个户居民食用盐进行定性检测。结果 调查8个县92个乡镇的451个村庄,检测水样800份、食盐800份。水碘含量均值为(110.93±152.26)μg/L,中位数为55.83μg/L(0.84~997.82μg/L);96.5%为平原地区水源。井深平均(70.44±136.19)m,中位数12.0m(2.0~999.0m)。水碘>150μg/L的高碘地区,分布于7个县的24个乡镇,受累人口102.39万人,碘盐覆盖率97.2%;其水碘含量为(327.72±192.19)μg/L,中位数253.87μg/L(150.78~997.82μg/L)。浅层井水的高碘含量可能与黄河泛滥把从上游携带来的富碘泥沙的长期沉积有关,而深井水高碘成因除此之外还与三角洲的沉积及海底大陆架千万年来沉积的富碘海洋生物有关。
  结论 黄河三角洲有24个高碘地区,碘盐覆盖率97.2%,既有浅井高碘水又有深井高碘水的独特现象。高碘地区应停止碘盐供应措施,开展健康教育和监测工作。

关键词:水碘 高碘地区 碘盐 黄河三角洲
中图分类号:R151.42 R123.1 R599.9 文献标识码:A

Study on the present status of the areas with high iodine concentration indrinking water and edible salt at household levels in Ohio of Yellow River

Guo Xiao - wei, Zhai Li - ping, Liu Yuan, Wang Xin,et al.

Shandong Institute for Endemic Disease Control and Research, Jinan 250014, China

Abstract : Objective To understand the present condition of iodine excess areas and edible salt at household levels in Ohio of Yellow River , which will provide the evidence to control it. Methods A cross section in one time was adopted for the epidemiological survey based on the east, west, south, north and central in all of townships from 8 counties. 2 samples of drinking water from each village were tested their water iodine content as well as the data regarding to their recourses and the depth of wells. 5 samples of edible salt were collected from each village for quantitative analysis. Results We investigated 451 villages in 92 townships of 8 counties. 800 samples of drinking water were tested which values of iodine content were (110 . 93 ± 152 . 26) μ g/L in main, 55 . 83 μ g/L(0 . 84 , 997 . 82 μ g/L)in medium. 102 . 39 thousand population are at risk for iodine excess and living in 24 townships of 7 counties where iodine concentration is over 150 μ g/L in drinking water, with (327 . 72 ± 192 . 19) μ g/L in mean value or 253 . 87 μ g/L(150 . 78 , 997 . 82 μ g/L)in medium. The rate of iodized salt is 97 . 2%. All the iodine excess areas are located in alluvial plain of Yellow River. The etiology of high iodine in shallow well water may be supposed to be iodine aggregation formed by Yellow River in terms of thousands of flood in thousands of years. But iodine excess in deep well water may be related to rotten, deposit marine living beings rich in iodine millions upon millions years ago. Conclusion There were distinctive features of iodine excess in drinking water from both shallow well and deep well, 24 iodine excess areas in Ohio of Yellow River. It has suggested that iodized salt intervention should be stopped in the areas and starting the health education project, survey of iodized salt in the region.

Key words: water iodine, iodine excess areas, iodized salt, Ohio of Yellow River

基金项目:国家自然科学基金重点项目( No. 30230330 );国际控制碘缺乏病理事会( ICCIDD );卫生部疾控司资助项目( 2003 )
作者简介:郭晓尉,男,副主 任 医师
1 天津医科大学内分泌研究所


 

沙门菌、志贺菌和大肠杆菌 O157 ∶ H7 的多重 PCR 快速检测体系的初步探讨
2006年8月17日

何超 樊学军1 汪东篱 刘丽英2 王翠苹2 裴晓方3
四川大学华西公共卫生学院,成都 610041


摘要:
  目的 建立能在12小时内同时快速检测沙门菌、志贺菌和大肠杆菌O157∶H7的多重PCR体系。
  方法 碱性蛋白胨水(BPW)非选择性增菌6h;100℃10min制备DNA模板;根据大肠杆菌O157∶H7的uidA基因、志贺菌的ipaH基因及沙门菌的invA基因序列设计各菌引物,进行PCR扩增及电泳检测。同时优化反应体系,测定体系灵敏度和特异性。
结果 该多重PCR体系能在12h内同时检测3种目的菌;灵敏度为10~30cfu/ml;通过对23株目的菌和15株非目的菌检测,提示该体系特异性高。
  结论 初步探讨出能在12h内快速、灵敏、特异地同时测定沙门菌、志贺菌和大肠杆菌O157∶H7的多重PCR体系。

关键词:多重PCR 沙门菌 志贺菌 大肠杆菌O157∶H7
中图分类号:R378.2 R155.31 文献标识码:A

Primarily study on a multiplex PCR based system for the rapid detection of Salmonella spp., Shigella spp. and Escherichia coli O157 H7

He Chao, Fan Xue - jun, Wang Dong - li, Liu Li - ying, et al.

West China School of Public Health, Sichuan University , Chengdu 610041, China

Abstract:Objective To establish a multiplex PCRbased system for the simultaneous detection of Salmonella spp., Shigella spp. and Escherichia coli O157∶H7 in 12 hours.Methods After 6 h nonselective enrichment in BPW, DNA template were prepared at 100℃ for 10min. Three sets of primers were designed to amplify the gene segments of invA of Salmonella spp, ipaH of Shigella spp, and uidA of E. coli O157∶H7, and the products were analyzed by electrophoresis. At the same time, this system was optimized,and the specificity and sensitivity of this system were evaluated.Results Three target bacteria were detected in 12 h by using this multiplex PCRbased system. The sensitivity of it was up to 10-30cfu/ml, and the high specificity was demonstrated by detecting 23 target stains and 15 nontarget stains.Conclusion A rapid,specific, and sensitive multiplex PCRbased system for the simultaneous detection of Salmonella spp., Shigella spp. and E. coli O157∶H7 in 12 h has been studied primarily.

Key words:multiplex PCR, Salmonella spp., Shigella spp, Escherichia coli O157∶H7

基金项目:国家质检总局科研计划项目( No.J2005J0115 )
作者简介:何超,女,硕士研究生
1 四川省检验检疫局国际旅行卫生保健中心
2 四川大学华西公共卫生学院 99 级卫检本科生
3 联系作者

 
不同体脂人群肠道主要菌群的定量分析

刘祥 张朝武 潘素华 裴晓方 余倩1
四川大学华西公共卫生学院,成都 610041

摘要:
  目的 探讨主要肠道菌群在不同体脂人群间数量变化及其对脂质代谢可能具有的影响。
  方法 照BMI分类标准,将研究对象分为消瘦组、正常组、超重组、肥胖组,采用选择性培养法测定研究对象新鲜粪便中5种主要肠道菌群的数量。结果 随着BMI值的升高,乳酸杆菌、双歧杆菌和肠杆菌数量呈下降趋势,拟杆菌数量则显著升高(P<0.01),肠球菌和产气荚膜梭菌的数量无明显的变化趋势。
  结论 拟杆菌对脂肪积累和肥胖形成可能具有一定的促进作用,而肠杆菌、乳酸杆菌和双歧杆菌则可能起相反的作用。

关键词:肥胖 BMI 肠道菌群 微生态
中图分类号:R378.2 R589.2 文献标识码:A

Quantitative analysis of dominating intestinal flora amonggroups of people with different body fat

Liu Xiang, Zhang Chao - wu, Pan Su - hua, Pei Xiao - fang, et al.

West China School of Public Health, Sichuan University , Chengdu 610041, China

Abstract : Objective To investigate the alteration of dominating intestinal floras among groups of people with different body fat and probe into the possible effect on lipid metabolism. Methods According to the BMI values, subjects were divided into 4 groups, including fleshless group, normal group, overweight group and obese group. Five dominating floras of all fresh stools were quantitated using selective culture method, and all data were analyzed statistically. Results With the increase of BMI values, there is a decreasing trend in the amount of  Lactobacillus, Bifidobacterium  and  Enterobacillus  , and the amounts of  Bacteriodes  obviously increased (P<0 . 01). No obvious alternation of the amounts of  Enterococcus  and  Clostridium  was observed. Conclusion Bacteriodes may exert an boosting effect on the pile of fat and development of obese, however Enterobacillus, Lactobacillus, and Bifidobacterium have a potential opposite effect.

Key words: obesity , BMI , intestinal flora , microecology

作者简介:刘祥,男,博士研究生
1:通讯作者